Fig. 4

Overexpression of MITF and PU.1 after day 2 of differentiation did not affect the adipogenesis of the ovine primary preadipocytes. The primary preadipocytes were induced to differentiate. On day 2 of differentiation after the induction solution 1 was changed for 4 h, pcDNA-MITF and pcDNA-PU.1 were transfected alone or co-transfected into the cells. Oil Red O staining and TG content analyses were applied to examine the lipid accumulation in the cells on day 6. a – Oil Red O staining for the cells on day 6 of differentiation. The scale bar is 200 μm. The bar diagram (bottom left) showing the OD value (510 nm) of the cell matrix containing ovine primary preadipocytes transfected with MITF siRNA and/or PU.1 siRNA on day 6 of differentiation. b – TG content analysis for cells on day 6 of differentiation (510 nm OD value). c – TG content analysis for cells on day 6 of differentiation (umol/ng protein). The same letters on the bar diagram indicated no significant differences between datasets; significant differences indicated by different letters. Significance was set at p < 0.05 (n = 3), *p < 0.05 vs. control, ^# p < 0.05 vs. siMITF. d. Expression of MITF, PU.1, C/EBPβ and PPARγ after pcDNA-MITF and/or pcDNA-PU.1 transfection