Fig. 4

TMEM43 mediates PRPF3 protein stability by directly binding to PRPF3. A Venn diagram showing the proteins regulated by TMEM43 and the proteins that directly interact with TMEM43. B IHC showing the correlation of TMEM43 protein expression and PRPF3 protein expression. C The correlation of PRPF3 mRNA levels with RAP2B mRNA levels in the GSE71729 dataset. D Endogenous binding of TMEM43 and PRPF3 was detected using co-IP and western blot assays. E Co-IP and western blot assays were used to analyze the exogenous binding of TMEM43 and PRPF3. F Confocal immunofluorescence assays detected the location of TMEM43 and PRPF3 in pancreatic cancer cells. G, H The PRPF3 protein and mRNA levels were detected in TMEM43-silenced MIAPaCa-2, SW1990 cells, overexpressing TMEM43 MIAPaCa-2 cells, and the corresponding control cells. I The protein levels of PRPF3 in TMEM43-silenced MIAPaCa-2 and SW1990 cells and the corresponding control cells were detected after treatment with the protein synthesis inhibitor cycloheximide (CHX, 25 µg/mL). Results are shown as the mean ± SD of three independent replicates. *p < 0.05, **p < 0.01, ***p < 0.001