Fig. 4

miR-221/222 mediated the regulation of METTL3 on Ang-II-induced cardiomyocyte hypertrophy. A miR-221 and miR-222 in NRCMs were inhibited by the specific inhibitors during Ang-II-induced hypertrophy. Representative immunohistochemistry images of NRCMs stained with α-actinin in green. The nucleus stained in blue. Scale bars, 10 μm. Cell surface area was evaluated. n = 3. B qRT-PCR detected the expression of hypertrophic biomarkers ANP and BNP in Ang-II-induced NRCMs transfected with inhibitors of miR-221/222. n = 3. C Western blot detected the expression of hypertrophic biomarkers ANP and BNP in Ang-II-induced NRCMs transfected with inhibitors of miR-221/222. n = 3. D miR-221 mimics and miR-222 mimics were transduced into METTL3-knockdown NRCMs and stimulated with Ang-II. Representative immunohistochemistry images of NRCMs stained with α-actinin in green. The nucleus is stained in blue. Scale bars, 10 μm. Cell surface area was evaluated. n = 3. E qRT-PCR detected the expression of biomarkers ANP and BNP in Ang-II-induced NRCMs transfected with shMETTL3 and miR-221/222 mimics. n = 3. F Western blot detected the expression of biomarkers ANP and BNP in Ang-II-induced NRCMs transfected with shMETTL3 and miR-221/222 mimics. n = 3. *P < 0.05, **P < 0.01, ***P < 0.001