Fig. 1
From: SESN2 prevents the slow-to-fast myofiber shift in denervated atrophy via AMPK/PGC-1α pathway
Denervation led to skeletal muscle atrophy and slow-to-fast myofiber shift. A Wet weight ratio (the weight of the operational side divided by the contralateral side) of GAS, EDL, and SOL at indicated timepoints post-denervation. B Western blot analysis of the dynamic changes of MHC protein expression after denervation. C, D Analysis of gastrocnemius fibers diameter by H&E and immunofluorescence staining. Scale bar, 50 μm. E Western blot analysis of proteins related to muscle fiber type. F Immunofluorescence analysis of the fiber type composition in denervated GAS. Scale bar, 50 μm. G Microstructure of GAS. Red arrows indicate mitochondria. Scale bar, 2 μm. H, I Relative ATP content and metabolic enzyme activity (LDH and SDH) in GAS at different timepoints post-denervation. Data presented as mean ± standard deviation (SD). n = 6. *P < 0.05 versus control (week 0)