Fig. 8From: LARRPM restricts lung adenocarcinoma progression and M2 macrophage polarization through epigenetically regulating LINC00240 and CSF1Ectopic expression of LARRPM in LUAD cells attenuated the oncogenic roles of infiltrated M2 macrophages. a–f CM was collected from co-culture of macrophages with LARRPM overexpressed or control A549 cells, or collected from only A549 cells with LARRPM overexpression or A549 control cells. a, b Cell proliferation of A549 cells treated with the CM was detected using the CCK-8 assay (a) and EdU incorporation assay (b). Scale bar: 100 µm. Red color indicates EdU-positive cells. c, d Cell apoptosis of A549 cells treated with the CM was detected using the caspase-3 activity assay (c) and TUNEL assay (d). Scale bar: 100 µm. Green color indicates TUNEL-positive cells. e, f Cell migration and invasion of A549 cells treated with the CM was detected using the transwell migration assay (e) and transwell invasion assay (f). Scale bar: 100 µm. Results are shown as the mean ± SD based on three independent experiments. Asterisks indicate a significant difference at *P < 0.05, **P < 0.01, ns, not significant, by Student’s t-test. CM Conditioned mediumBack to article page