Fig. 3

Inhibition of autophagy hinders the process of ferritinophagy and ferroptosis induced by CISD2 knockdown. A Cell survival assay in control or CISD2-silenced HT-1080 cells under Erastin treatment (7.5 µM) with or without BafA1 (40 nM) co-treatment. B Western blot analysis of FTH expression on the condition of autophagy inhibition; GAPDH was used as loading control. C, D Representative images from flow cytometry for the detection of free iron and lipid peroxides by the staining of RPA and BODIPY, respectively; the intensity of RPA fluorescence was negatively correlated with iron accumulation, and the intensity of BODIPY fluorescence was positively correlated with lipid peroxides. ^★P < 0.05 and ^★★P < 0.01 between the indicated groups