Fig. 8

Inhibition of miR-103a-3p triggers EVA1A-dependent mitochondrial dysfunction. A, B Hccl-M3 cells were transfected with miR-103a-3p inhibitor or siRNA of EVA1A, or both of them. The changes of mitochondrial membrane potential were evaluated by JC-1 staining. C, D Intracellular ROS levels were determined by DCFH-DA probes. Scale bars, 200 μm. E Intracellular ATP levels were detected by the ATP Assay Kit. Data are presented as means ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001