Fig.4

Hsa-miR-7-5p targets the RYK gene. A The wild type (WT) and mutant (MUT) of miR-7-5p at the binding site of the 3′ UTR of the RYK gene. B Validation of miR-7-5p targeting to RYK using luciferase reporter gene assays. MDA-MB-231 cells were co-transfected with negative control (NC) or miR-7-5p mimics and luciferase reporter plasmids (including RYK 3′ UTR WT or RYK 3′ UTR MUT). C Expression of RYK mRNA in MDA-MB-231 cells transfected with NC or miR-7-5p mimics and inhibitors was measured by quantitative real-time PCR. D Western blot analysis of RYK protein expression in MDA-MB-231 cells transfected with NC or miR-7-5p mimics and inhibitors. E Wound healing experiments using blank control, siRYK and siRYK + miR-7-5p inhibitor after co-treatment of MDA-MB-231 cells. F A Transwell assay was used to verify the migratory invasion ability of MDA-MB-231 cells treated with blank control, siRYK and siRYK + miR-7-5p inhibitor. Data are presented as mean ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001