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Fig. 3 | Cellular & Molecular Biology Letters

Fig. 3

From: ALKBH5 in mouse testicular Sertoli cells regulates Cdh2 mRNA translation to maintain blood–testis barrier integrity

Fig. 3

Destroyed integrity of the blood–testis barrier in mice with Alkbh5 knockdown in Sertoli cells mediated by AAV8 infection. a Images showing GFP fluorescence in the testes infected with AAV8-scramble-GFP. b Immunofluorescence analysis to confirm the specificity of AAV8 infection in Sertoli cells (n = 3). DDX4, marker for germ cells but nonspermatid; SOX9, Sertoli cell marker; PNA, spermatid marker. c BTB assay detecting the integrity of the blood–testis barrier affected by AAV-mediated Alkbh5 knockdown specifically in mouse Sertoli cells

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