Fig. 3From: ALKBH5 in mouse testicular Sertoli cells regulates Cdh2 mRNA translation to maintain blood–testis barrier integrityDestroyed integrity of the blood–testis barrier in mice with Alkbh5 knockdown in Sertoli cells mediated by AAV8 infection. a Images showing GFP fluorescence in the testes infected with AAV8-scramble-GFP. b Immunofluorescence analysis to confirm the specificity of AAV8 infection in Sertoli cells (n = 3). DDX4, marker for germ cells but nonspermatid; SOX9, Sertoli cell marker; PNA, spermatid marker. c BTB assay detecting the integrity of the blood–testis barrier affected by AAV-mediated Alkbh5 knockdown specifically in mouse Sertoli cellsBack to article page