Fig. 1

The global cell types and changes in mice liver tissues delineated by scRNA-seq. A An overview of the study design. B The H&E staining of liver tissue (scale bar, 100 μm). Note the fibroblast proliferation in the Glisson’s capsule (black arrow), exudation (green arrow), slight inflammatory cell infiltration (yellow arrow), and focal necrosis (blue arrow) in GLY-treated mice. C UMAP plot displaying 13 major cell clusters based on 60,348 single-cell transcriptomes. D Violin plots showing the expression of two well-established marker genes for each cell type. E Distribution comparison of clusters from control (CON) and GLY groups. F Percent contribution of control (blue) and GLY (orange) mouse liver cells for each cell type. Note that the relative percentages of HSCs and LCMs were significantly increased by GLY. G The distributions of upregulated and downregulated genes for each cell type after GLY treatment. Note that HSCs and LCMs contained the largest number of DEGs. H The number of upregulated and downregulated genes for all cells after GLY treatment. Note that HSCs and LCMs contained the largest number of DEGs