Fig. 1

IC and IR downregulated the expression of miR-92a and increased apoptosis and autophagy in mice. A The content of serum Cr and BUN in the sham and IR48 groups. BThe expression of miR-92a. Representative band C and statistical analysis D of LC3B II/I and active caspase 3 expression using Western blotting. E Representative images of H&E staining (×100), TUNEL (×100), and TEM (×2000) in each group. F The statistical results of autophagosomes in I, IC, and IR groups. Using Western blotting, the representative band G and statistical analysis H of LC3B II/I, Beclin 1, pJNK, JNK, MEK4, and active caspase 3 expression. *P < 0.05. BUN blood urea nitrogen, Cr creatinine, GAPDH glyceraldehyde phosphate dehydrogenase, HE hematoxylin–eosin, I ischemia, IC ischemia and cold preservation, IRI ischemia and reperfusion injury, JNK c-Jun NH (2) terminal kinase, LC3B microtubule-associated protein 1 light chain 3B, MEK4 mitogen-activated protein kinase, pJNK phosphorylated JNK, TEM transmission electron microscope, TUNEL terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling