Fig. 3

Regulation of autophagy and apoptosis in HK-2 cells by miR-92a.The apoptotic rate A and the number of autophagosomes B of HK-2 cells in each group were compared. C Luciferase reporter assay found that miR-92a agomir targeted 3′-UTR of MEK and JNK. Representative band and statistical analysis of regulating miR-92a on the protein expression D of hypoxia and reoxygenation groups and the protein expression E of Rapa-treated groups. *P < 0.05. Con control, GAPDH glyceraldehyde phosphate dehydrogenase, Hypo hypoxia, LC3B microtubule-associated protein 1 light chain 3B, NC negative control, pJNK phosphorylated c-Jun NH (2) terminal kinase, Rapa rapamycin, Reo reoxygenation, 25ag 25 nM miR-92a agomir, 50an 50 nM miR-92a antagomir