Fig. 1

U73122 inhibited the effect of INSL3 on the proliferation of gubernacular cells. A Cell morphology was observed by HE staining. Scale bars, 50 μm. B Cell proliferation was detected by CCK8. C Cell counting measures changes in the number of cells. The counting method is shown in Additional file 2: Fig. S2. D The expression of proliferating cell nuclear antigen (PCNA) was observed by immunofluorescence. Scale bars, 20 μm. E The expression of PCNA protein was analyzed by Western blot. Data are mean ± standard deviation of three independent experiments. *^&#P < 0.05, **^&##P < 0.01, and ***^&###P < 0.001