Fig. 7

Aggregation of ATP8A2 in the HCs of P7 mouse organ of Corti. a–d At P1, ATP8A2 distributed in the cytoplasm of HCs in organ of Corti in both WT (a, b) and TMEM30A KO (c, d) mice. e, f ATP8A2 expression in cytoplasm diminished and more concentrated at the cuticular plate in WT organ of Corti at P7. g, h In TMEM30A KO cochlea, the ATP8A2 pattern was similar to that in P1 cochlea, suggesting the failed translocation to the proper subcellular structure without TMEM30A. i Quantitative analysis of ATP8A2 fluorescence intensity in hair cell cytoplasm. Red ATP8A2, blue DAPI. Scale bar, 20 µm. Error bars indicate the standard error of the mean. In i, N = 5 or 6