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Fig. 4 | Cellular & Molecular Biology Letters

Fig. 4

From: rt269L-Type hepatitis B virus (HBV) in genotype C infection leads to improved mitochondrial dynamics via the PERK–eIF2α–ATF4 axis in an HBx protein-dependent manner

Fig. 4

Comparison of autophagy/mitophagy induction in genotype A and C HBV infection. A EGFP-LC3 assay by flow cytometry. EGFP-LC3B-positive HepG2 cells cotransfected with mock, rt269L (genotype A or C), or rt269I (genotype A or C) vectors were detected by flow cytometry. B Detection of LC3 puncta after transfection of the EGFP-LC3 vector with genotype A or C vectors. LC3 is recruited to autophagosomes, forming a punctate structure, as shown by green dots. C Immunofluorescence for the colocalization of autophagosomes with mitochondria. The EGFP-LC3-expressing vector was cotransfected with HBV genome plasmids. Mitochondria were stained with MitoTracker deep red, and yellow dots represent colocalized EGFP-LC3-positive autophagosomes with red-labeled mitochondria. D Western blot analysis of the mitophagy marker protein PINK1. The relative intensity of PINK1/GAPDH staining was analyzed. **p < 0.01, ***p < 0.001

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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