Fig. 3

CircRNF10 interacted with DHX15 in the cytoplasm of BC cells. A Silver staining of the products of RNA pulldown in BC cells by the probe of circRNF10. Red arrow: differential protein band. B Mass spectrometry results of the differential protein band. C Western blotting analysis of the products of RNA pulldown. D RT-PCR analysis of the products of RIP assay in BC cells. E RT-qPCR analysis of the products of RIP assay. F FISH combined with IF to detect the colocalization of circRNF10 and DHX15 in BC cells. Red: circRNF10, green: DHX15, blue: DAPI. White arrows marked the colocalization of circRNF10 and DHX15. G Schematic illustration of the GFP-labeled truncations of DHX15. N-ext: N-extension of DHX15, C-term: C-terminal of DHX15. H Western blotting analysis of the products of RNA pulldown in HEK293T cells, which expressed exogenous DHX15 truncations. I RT-PCR analysis of the products of RIP assay in HEK293T cells that expressed exogenous DHX15 truncations. J RT-qPCR analysis of the products of RIP assay in HEK293T cells that expressed exogenous DHX15 truncations. K Western blotting analysis of the products of RNA pulldown in HEK293T cells that expressed GFP-DHX15-MUT (P327E, T421A, N422K, and Y485E). L RT-PCR analysis of the products of RIP assay in HEK293T cells that expressed GFP-DHX15-MUT (P327E, T421A, N422K, and Y485E). M RT-qPCR analysis of the products of RIP assay in HEK293T cells that expressed GFP-DHX15-MUT (P327E, T421A, N422K, and Y485E). Error bars represent the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns: no significance