Fig. 6
From: Hsp70-Bim interaction facilitates mitophagy by recruiting parkin and TOMM20 into a complex
S1g-2 or parkin shRNA enhanced cytochrome c release induced by HBSS. A Western blot analysis of the LC3 level in isolated mitochondria from NS shRNA-transfected or parkin shRNA-transfected HEK293T treated with HBSS. B Western blot analysis of cytochrome c in isolated mitochondria and cytosol supernatant from HEK293T cells or parkin shRNA-transfected cells after treatment with HBSS and 5 μM S1g-2 alone or in combination for 24 and 48 h respectively. An equivalent of DMSO was added to the compound untreated group as vehicle control. All figures represent the results from n = 3 biologically independent experiments. **P < 0.01 (two-way ANOVA test)