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Fig. 1 | Cellular & Molecular Biology Letters

Fig. 1

From: PRDX1 negatively regulates bleomycin-induced pulmonary fibrosis via inhibiting the epithelial-mesenchymal transition and lung fibroblast proliferation in vitro and in vivo

Fig. 1

Effect of PRDX1 on BLM-induced viability, ROS levels, and epithelial cell migration. Besa-2B cells were treated with 10 μg/mL BLM for 72 h (panels D–I). A PRDX1 protein levels were examined by western blot. B Cell viability was estimated by MTT assay. C The level of intracellular ROS was estimated by flow cytometry. D The levels of intracellular ROS was monitored by DHE fluorescence photography. E The levels of mitochondrial ROS were measured using Mito-SOX. F Mitochondrial membrane potential was determined using JC-1. G The morphology of cellular fibrosis was observed under microscope. H Cell migration was estimated by wound healing assay. I Quantitative analysis of data presented in panel H. *, p < 0.05; **, p < 0.01; ***, p < 0.001. PRDX1 peroxiredoxin, BLM bleomycin, ROS reactive oxygen species, MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, DHE dihydroethidium, Mito-SOX mitochondrial superoxide

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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