Fig. 3

Effect of PRDX1 on TGF-β secretion and oxidative stress in primary pulmonary fibroblasts. FMLF were treated with 10 μg/mL BLM for 72 h (panels C–F). A Cell viability was estimated by MTT assay. B TGF-β1 secreted by the cells was quantified using an ELISA kit. C The levels of intracellular ROS were measured using DHE. D The ROS levels in the mitochondria were determined using Mito-SOX. E The mitochondrial membrane potential was measured using JC-1. F Cell migration was estimated by wound healing assay. *, p < 0.05; **, p < 0.01; ***, p < 0.001. PRDX1 peroxiredoxin, TGF-β1 transforming growth factor β1, FMLF foetal mouse lung fibroblasts, BLM bleomycin, MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, TGF-β1 transforming growth factor β1, ELISA enzyme-linked immunosorbent assay, ROS reactive oxygen species, DHE dihydroethidium, Mito-SOX mitochondrial superoxide