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Fig. 4 | Cellular & Molecular Biology Letters

Fig. 4

From: PRDX1 negatively regulates bleomycin-induced pulmonary fibrosis via inhibiting the epithelial-mesenchymal transition and lung fibroblast proliferation in vitro and in vivo

Fig. 4

Effects of PRDX1 on proliferation, collagen-associated protein expression, and the PI3K/Akt and JNK/Smad signalling pathways in primary pulmonary fibroblasts. FMLF were treated with 10 μg/mL BLM. A Cell viability was estimated by MTT assay. B The cell cycle stage was estimated by flow cytometry. C The expression levels of cell cycle-related proteins. D The expression levels of fibrosis-related proteins. E Sample correlation was analysed by Pearson correlation coefficient and principal components analysis. F Differentially expressed genes in volcano mapping. G Cluster analysis of differentially expressed genes. H The expression levels of PI3K/Akt and JNK/Smad signalling pathways-related proteins. Protein levels were determined by western blot analysis. *, p < 0.05; **, p < 0.01; ***, p < 0.001. PRDX1 peroxiredoxin, PI3K phosphoinositide 3-kinase, Akt protein kinase B, JNK c-Jun N-terminal kinase, Smad small of mothers against decapentaplegic, FMLF foetal mouse lung fibroblasts, BLM bleomycin, MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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