Fig. 2
Upregulation of S100a9 expression participates in β1-AA-induced autophagy inhibition in cardiomyocytes. A LC3 and p62 protein expression in C57BL/6 mice actively immunized for 4 weeks were detected by western blot (n = 6 per group). B The levels of LC3 and p62 in cardiomyocytes stimulated with β1-AAs were detected by western blot (n = 4 per group). C, D The mRNA levels of S100a9 and LC3 in H9c2 cells stimulated with β1-AAs after transfection with S100a9 siRNA or NC siRNA were detected by RT-PCR (n = 6 per group). E S100a9, LC3, and p62 protein expression in H9c2 cells treated with β1-AAs after transfected with S100a9 siRNA or NC siRNA were detected by western blot (n = 6 per group). F Statistical diagram of S100a9, LC3, and p62. Data are presented as the mean ± SEM; *p < 0.05 vs. control, **p < 0.01 vs. control, #p < 0.05 vs. β1-AA group, ##p < 0.01 vs. β1-AA group