Fig. 3

Upregulation of S100a9 inhibits Atg9a transcription, which leads to inhibition of autophagy in cardiomyocytes. A Relative mRNA expression levels of 30 autophagy-related genes in cardiomyocytes. B Atg9a mRNA was assayed by RT-PCR after transfection with S100a9 siRNA or NC siRNA. C Atg9a mRNA was detected by RT-PCR after transfection with the S100a9 plasmid. D Atg9a protein expression was measured by western blot. E Atg9a mRNA in H9c2 cells treated with β₁-AAs after transfection with S100a9 siRNA or NC siRNA was assayed by RT-PCR. F Western blot analysis for determining the level of Atg9a in H9c2 cells treated with β₁-AAs after transfection with S100a9 siRNA or NC siRNA. G The expression level of LC3 in cardiomyocytes transfected with the S100a9 plasmid and Atg9a plasmid was detected by RT-PCR. H LC3 and p62 protein expression in H9c2 cells transfected with an S100a9 plasmid and Atg9a plasmid was detected by western blot. Cardiomyocytes were stimulated with β₁-AAs for 24 h. Data are presented as the mean ± SEM, with n = 6 per group; *p < 0.05 vs. control, **p < 0.01 vs. control. E and F ^#p < 0.05 vs. β₁-AA group, ^##p < 0.01 vs. β₁-AA group. G and H ^#p < 0.05 vs. S100a9 overexpression group, ^##p < 0.01 vs. S100a9 overexpression group