Fig. 4
β1-AA-induced upregulation of HIF-1α did not promote transcription of Atg9a. A JASPAR analysis (http://jaspar.genereg.net/) revealed the recognition sites of HIF-1α on the promoter sequence of Atg9a. B Detection of luciferase activity after the Atg9a promoter sequence plasmid and HIF-1α plasmid were transfected into H9c2 cells. C Atg9a mRNA level was assayed by RT-PCR. D) Atg9a protein expression was detected by western blot. E Western blot analysis for determining the HIF-1α level after β1-AA treatment in whole cell lysates. F Double-luciferase reporter gene assay of cardiomyocytes treated with β1-AAs was used to detect the luciferase activity of Atg9a promoter. Data are presented as the mean ± SEM with n = 6 per group; *p < 0.05 vs. control, **p < 0.01 vs. control, and #p < 0.05 vs. Luc-Atg9a + HIF-1α group