Fig. 5

Combination of S100a9 and HIF-1α prevented HIF-1α from entering the nucleus. A, B Immunoprecipitation showed the interaction between S100a9 and HIF-1α in cardiomyocytes treated with β₁-AAs for 24 h. C Immunofluorescence was used to detect the nuclear translocation and cytoplasmic co-localization of HIF-1α after β₁-AAs administration for 24 h via immunofluorescence. The white arrows indicate HIF-1α protein in the nucleus. The yellow arrows indicate cytoplasmic co-localization of the HIF-1α protein and S100a9. D The distribution of HIF-1α in the cytoplasm and nucleus in cardiomyocytes incubated with β₁-AAs for 24 h was detected. Data are presented as the mean ± SEM with n = 6 per group; *p < 0.05 vs. control, **p < 0.01 vs. control