Fig. 2

RGS6 was downregulated in ALI models in vivo and in vitro. A HE staining showed pathological changes in lung tissue after LPS administration. Scale bar: 100 μm. B RGS6 mRNA relative expression levels in lung tissue after LPS administration. C Western blotting showed RGS6 protein expression in lung tissue after LPS administration. D Quantitative analysis showed RGS6 protein relative expression levels in lung tissue in PBS and LPS group. E. RGS6 mRNA relative expression levels in RAW264.7 cells after LPS administration (100 ng/ml for 8 h). F Western blotting showed RGS6 protein expression levels in RAW264.7 cells after LPS administration. G RGS6 mRNA relative expression levels in MLE-12 cells after LPS administration (1ug/ml for 24 h). H Western blotting showed RGS6 protein expression levels in MLE-12 cells after LPS administration. I RGS6 mRNA relative expression levels in lung tissue 3 days and 10 days after LPS administration. Data are shown as mean ± SEM. PBS, wild type mice given PBS intratracheally and executed at day 3. LPS, wild type mice given LPS (5 mg/kg) intratracheally and executed at day 3. C: cells without LPS stimulation. LPS: cells with LPS stimulation. ^***P < 0.001 vs. PBS group or C group; ^#P < 0.05 vs. LPS-Day 3 group