Fig. 4

RGS6 knockout promoted inflammation and apoptosis in the lungs of ALI models. A Immunohistochemistry staining of Ly6g showed neutrophils infiltration in different groups. Scale bar: black 500 μm, red 50 μm. B Tunnel staining showed cell apoptosis in different groups. Cell nucleus was stained by DAPI (blue fluorescence). Apoptotic cells were stained by TUNEL (green fluorescence). Scale bar: white 50 μm. C Western blotting showed MPO expression levels of lung tissue in different groups. D Quantitative analysis for MPO relative protein expression levels in lung tissue. E ELISA detected IL-6 concentration in BALF in different groups. F ELISA detected IL-1β concentration in BALF in different groups. G ELISA detected MCP-1 concentration in BALF in different groups. WT PBS, wild type mice given PBS intratracheally and executed at day 3; WT LPS, wild type mice given LPS (3 mg/kg) intratracheally and executed at day 3; RGS6^−/− LPS, RGS6^−/− mice given LPS (3 mg/kg) intratracheally and executed at day 3. Data are shown as mean ± SEM. ^*P < 0.05 vs. WT PBS group; ^***P < 0.001 vs. WT PBS group; ^#P < 0.05 vs. WT LPS group; ^###P < 0.001 vs. WT LPS group