Fig. 5

RGS6 knockout reduced the regenerative capacity of AEC2s. A qRT-PCR showed SFTPC mRNA relative expression levels in lung tissue in different groups. B qRT-PCR showed SCGB1A1 mRNA relative expression levels in lung tissue in different groups. C Immunohistochemistry showed AEC2 loss in different groups. AEC2s were stained with anti-SFTPC antibody. Scale bar: 50 μm. WT PBS, wild type mice given PBS intratracheally and executed at day 3; WT LPS, wild type mice given LPS (3 mg/kg) intratracheally and executed at day 3; RGS6^−/− LPS, RGS6^−/− mice given LPS (3 mg/kg) intratracheally and executed at day 3. Data are shown as mean ± SEM. ^*P < 0.05 vs. WT PBS group; ^**P < 0.01 vs. WT PBS group ^##P < 0.01 vs. WT LPS group. ^ns, no significance vs. WT LPS group