Fig. 7

RGS6 overexpression in macrophage decreased intracellular ROS production and proinflammatory cytokine transcription. A Structural map of RGS6 pcDNA3.1(+). B RGS6 overexpression efficiency was shown by qRT-PCR. Control group was flagged as NC, while RGS6 overexpression group was flagged as OE. C Western blotting showed overexpression of RGS6 protein in RAW264.7 cells. D Flow cytometry showed intracellular ROS production in RAW264.7 cells. E Quantitative analysis showed mean fluorescence intensity about DCFH-DA-FITC in RAW264.7 cells. Proinflammatory cytokines iNOS (F), IL-1β (G), IL-6 (H), TNF-α (I) and MCP-1 (J) were detected by qRT-PCR. NC-C, RAW264.7 cells transfected with control plasmid for 24 h followed by culturing for another 8 h. OE-C, RAW264.7 cells transfected with RGS6 overexpression plasmid for 24 h followed by culturing for another 8 h. NC-LPS, RAW264.7 cells transfected with control plasmid for 24 h followed by stimulating with LPS (100 ng/ml) for another 8 h. OE-LPS, RAW264.7 cells transfected with RGS6 overexpression plasmid for 24 h followed by stimulating with LPS (100 ng/ml) for another 8 h. Data are shown as mean ± SEM. *P < 0.05 vs. NC-C group; **P < 0.01 vs. NC-C group; ***P < 0.001 vs. NC-C group. ^#P < 0.05 vs. NC-LPS group; ^##P < 0.01 vs. NC-LPS group. ^###P < 0.001 vs. NC-LPS group