Fig. 1

Significant increase of MAMs was observed in MT-induced hepatic injury. A Serum ALT levels at different time points. B Serum AST levels at different time points. C Representative TEM images at different time points at × 15,000 magnification. Scale bars: 1 μm. Pictures in bottom side are the magnified ones from top side, indicated by black frame. M: mitochondria; ER: Endoplasmic reticulum; Red arrow: minimum ER-mitochondria distance. D Quantification of the minimum distance between ER and mitochondria. E Quantification of ER length close to mitochondria normalized by total ER length at different time. All of the values are shown as the means ± SEM. n = 8 in each group. *P < 0.05, **P < 0.01 vs Sham; ^{^^}P < 0.01 vs 0 h after MT