Fig. 4
Melatonin inhibited cell death and MAMs formation in the hepatocytes cultured in TS. A Cell viability (percentage of SS). B, C Representative blots and quantitative analysis of IP3R1, cleaved-caspase 3 (n = 4). D Relative activity of caspase 3. E Representative confocal images of primary hepatocytes double-stained by mitotracker (red) and ER-localized virus (green) at × 600 magnification. F, G The representative figures of scatter plot were analyzed by colocalization finder in Image J software. Statistical quantification of the colocalization area between mitochondria and ER. H Representative images and quantitative analysis of MitoSOX-stained mitochondria-derived superoxide production. All of the values are shown as the means ± SEM. n = 6 in each group. **P < 0.01 vs SS; ##P < 0.01 vs TS