Fig. 2

Identification of circRNA3634 in antler chondrocytes. A Prediction of the circRNA-miR-124486-5-MAPK1 axis. B qRT-PCR was performed to measure the expression of circRNAs in mesenchyme and cartilage tissues. C Schematic diagram illustrating that circRNA3634 is formed through the back-splicing of COG3 pre-mRNA. Sanger sequencing and agarose gel electrophoresis confirmed the back-splicing site. D Expression of circRNA3634 and COG3 mRNA assessed after treatment with RNase R. E Levels of circRNA3634 and COG3 mRNA determined by qRT-PCR following Act D treatment. F FISH experiments were conducted to detect circRNA3634 in antler chondrocytes. Nuclei were stained with DAPI (blue) and circRNA3634 probes were labeled with Cy3 (red). Scale bar, 15 μm. *p < 0.05, **p < 0.01. N.S., no signification