Fig. 3

CircRNA3634 promotes the proliferation and differentiation of antler chondrocytes. A qRT-PCR revealed significant upregulation of circRNA3634 in antler chondrocytes following transfection with pcDNA3.1-circRNA3634. B Relative expression of circRNA3634 and COG3 mRNA after transfection of NC or circRNA3634 siRNA into antler chondrocytes, determined by qRT-PCR. C–F CCK-8 and EdU assays showed that OE-circRNA3634 promotes the proliferation of antler chondrocytes and si-circRNA3634 inhibits it. Scale bar, 100 μm. G and H A cell migration assay indicated that OE-circRNA3634 stimulated antler chondrocytes migration and si-circRNA3634 inhibited it. Scale bar, 50 μm. I OE-circRNA3634 increased ALP activity and si-circRNA3634 decreased it. J OE-circRNA3634 intensified ALP staining and si-circRNA3634 attenuated it. K and L Relative mRNA expression of MAPK1, RAF1, MEK1, RUNX2, and SOX9 in antler chondrocytes transfected with pcDNA3.1-circRNA3634 or circRNA3634 siRNA, determined by qRT-PCR. M–P Western blots of the protein expression of p-ERK1/2, t-ERK1/2, RAF1, MEK1, RUNX2, and SOX9 in antler chondrocytes transfected with pcDNA3.1-circRNA3634 or circRNA3634 siRNA. Q Immunofluorescence image showing the expression of t-ERK1/2 in antler chondrocytes transfected with pcDNA3.1-circRNA3634 or circRNA3634 siRNA. Scale bar, 50 μm. *p < 0.05, **p < 0.01. N.S., no signification