Fig. 4

CircRNA3634 serves as a miR-124486-5 “sponge” in antler chondrocytes. A and B An RIP-PCR assay showed significant enrichment of circRNA3634 and miR-124486-5 in the AGO2 antibody immunoprecipitated complex. C Schematic diagram of the wild-type/mutant circRNA3634 dual-luciferase reporter vector. D Relative luciferase activity in antler chondrocytes co-transfected with the wild-type/mutant circRNA3634 dual-luciferase reporter vector and NC/miR-124486-5 mimic for 48 h. E Schematic diagram of the wild-type/mutant MAPK1 dual-luciferase reporter vector. F Relative luciferase activity in antler chondrocytes co-transfected with the wild-type/mutant MAPK1 dual-luciferase reporter vector and NC/miR-124486-5 mimic for 48 h. *p < 0.05, **p < 0.01. N.S., no signification