Fig. 5

miR-124486-5 inhibits antler chondrocyte proliferation and migration by targeting the 3ʹ UTR of MAPK1. A Less miR-124486-5 expression was observed in antler cartilage than in mesenchyme tissue. B and C Fold changes in miR-124486-5 expression in antler chondrocytes transfected with the miR-124486-5 mimic and inhibitor. D–I Effects of the miR-124486-5 mimic and inhibitor on antler chondrocytes, determined by CCK-8, EdU, and cell migration assays. Scale bars, 100 (F) and 50 (H) μm. J–M qRT-PCR, Western blot, and immunofluorescence analysis of MAPK1 mRNA, t-ERK1/2 and p-ERK1/2 protein expression in antler chondrocytes transfected with the miR-124486-5 mimic or inhibitor. Scale bar, 50 μm. *p < 0.05, **p < 0.01. N.S., no signification