Fig. 6

circRNA3634 regulates antler chondrocytes proliferation and migration via miR-124486-5. A–C Proliferation of antler chondrocytes co-transfected with pcDNA3.1-circRNA3634 and the miR-124486-5 mimic, determined by CCK-8 and EdU assays. Scale bar, 100 μm. D and E Cell migration assay results for antler chondrocytes co-transfected with pcDNA3.1-circRNA3634 and the miR-124486-5 mimic. Scale bar, 50 μm. F–I qRT-PCR, western blot, and immunofluorescence results showing that the decreases in MAPK1 mRNA, t-ERK1/2, and p-ERK1/2 protein expression caused by the miR-124486-5 mimic were partially rescued by OE-circRNA3634. Scale bar, 50 μm. *p < 0.05, **p < 0.01. N.S., no signification