Fig. 7

METTL3 reduces the stability of circRNA3634. A catRAPID prediction results of RBPs of circRNA3634. B SRAMP prediction of potential m6A sites on circRNA3634. C and D MeRIP-PCR and RIP-PCR demonstrated significant enrichment for circRNA3634 in m6A and METTL3 antibody precipitation complex enrichment. E RAP identification of the circRNA3634-protein enriched by circRNA3634 junction probe. F qRT-PCR measurement of METTL3 mRNA expression in antler chondrocytes transfected with METTL3 siRNA#1/2/3. G Western blot showing METTL3 protein expression in antler chondrocytes transfected with METTL3 siRNA. H and I RIP-PCR and MeRIP-PCR showed decreased enrichment of circRNA3634 in the m6A antibody precipitation complex after METTL3 siRNA transfection. J and K Reduced stability and expression of circRNA3634 after METTL3 siRNA transfection, detected by qRT-PCR. L qRT-PCR showed decreased expression of MAPK1, RAF1, MEK1, RUNX2, and SOX9 after METTL3 siRNA transfection. M CCK-8 analysis of the cell proliferation ability in antler chondrocytes transfected with METTL3 siRNA. Scale bars, 100 μm. N and O Cell migration assay results for antler chondrocytes transfected with METTL3 siRNA. Scale bar, 50 μm. P and Q ALP activity assay and ALP staining to detect si-METTL3 effects on antler chondrocyte differentiation. *p < 0.05, **p < 0.01. N.S., no signification