Fig. 8

YTHDC1 promotes the nuclear export of m6A-modified circRNA3634. A RIP-PCR showed the enrichment of circRNA3634 in the YTHDC1 antibody precipitation complex. B qRT-PCR measurement of YTHDC1 mRNA expression in antler chondrocytes transfected with YTHDC1 siRNA#1/2/3. C Western blot showed decreased YTHDC1 protein expression in antler chondrocytes transfected with YTHDC1 siRNA. D RIP-PCR showed decreased enrichment of circRNA3634 in the YTHDC1 antibody precipitation complex after YTHDC1 siRNA transfection. E FISH revealed the predominantly nuclear localization of circRNA3634 upon YTHDC1 siRNA transfection. Scale bar, 15 μm. F qRT-PCR showed the reduced expression of circRNA3634 in antler chondrocytes following YTHDC1 siRNA transfection. G, H Expression of MAPK1 mRNA and proteins of p-ERK1/2 and t-ERK1/2 in antler chondrocytes transfected with YTHDC1 siRNA was detected by qRT-PCR and western blot. I p-ERK1/2 relative ratio. J CCK-8 analysis of the cell proliferation ability in antler chondrocytes transfected with YTHDC1 siRNA. Scale bars, 100 μm. K and L Cell migration assay results for antler chondrocytes transfected with YTHDC1 siRNA. Scale bar, 50 μm. N–O ALP activity assay and ALP staining to detect si-YTHDC1 effects on antler chondrocyte differentiation. *p < 0.05, **p < 0.01. N.S., no signification