Fig. 2

Effects of miR-675-5p/miR-1247-5p on pyroptosis/neuroinflammation in PD models. The putative miR-675-5p binding site in the 3′ UTR of GSDME and relative luciferase activity was determined by luciferase reporter assay in SH-SY5Y cells co-transfected with miR-675-5p mimics/mimics-NC and wild reporter vectors (GSDME WT) (A). The putative miR-1247-5p binding site in the 3′ UTR of HMGB1 and relative luciferase activity was determined by luciferase reporter assay in SH-SY5Y cells co-transfected with miR-1247-5p mimics/mimics-NC and wild reporter vectors (HMGB1 WT) (B). The expression levels of HMGB1, GSDME, il-18, il-1β, NLRP3 and il-6 were measured by RT-qPCR. The delta-delta cycle threshold value (2^-ΔΔCt) method was used to analyze the results relative to GAPDH. The results were compared with control groups. Significant differences were assessed using a two-tailed student t-test. Data are represented as the mean ± SEM of three biological replicates performed using three technical replicates. (Ctl, control; SH, SH-SY5Y cell; α syn, α-synuclein; NM-Sal, NM-Salsolinol; miR, microRNA;*P < 0.05; **P < 0.01;***P < 0.001)