Fig. 2

HDAC9 is decreased in degenerated IVD in aged mice. A MRI scan of the lumbar spine and intervertebral discs (IVDs) of 6-month and 18-month-old mice. B Coronal sections of disc compartments stained by safranin-O/fast green. Scale bar: black, 500 μm; yellow, 200 μm. C Fluorescence staining of HDAC9 in disc compartments. Scale bar: White, 100 μm; Yellow, 50 μm. Quantification of staining for HDAC9-positive cells. Data are represented as mean ± SD (n = 6). A p-value of less than 0.05 was considered significant using an unpaired student’s t-test. NS: not significant