Fig. 4
From: Molecular mechanism and therapeutic potential of HDAC9 in intervertebral disc degeneration
mRNA-seq analysis determines HDAC9-regulated genes in NP cells. A Schematic of the NP cells isolated from HDAC9KO and HDAC9WT mice (n = 3) were subject to mRNA-seq. B Volcano plot of expression of genes. Green, downregulated DEGs; red, upregulated DEGs; blue, not significant. C Heatmap of the expression profiling of the top 20 upregulated and downregulated DEGs. The red color and green color represent upregulated DEGs and downregulated DEGs, respectively. D Schematic of GSEA enrichment analysis of genes from mRNA-seq. GSEA plot depicting the enrichment of genes in deubiquitinase activity, PI3K-Akt signaling pathway, muscle cell proliferation, deacetylase activity, cytokine–cytokine receptor interaction, oxidative phosphorylation, histone acetyltransferase activity, glycolysis/gluconeogenesis pathway, and modification-dependent protein binding