Fig. 6

Deficiency of HDAC9 affects cell apoptosis, viability, and regulates RUNX3 in NP tissue. A, B Fluorescence staining and quantification of percentage of Cyclin D1 positive cells, p21 positive cells, p53 positive cells, and RUNX3 positive cells in the NP tissues of 6-month-old HDAC9^WT mice and HDAC9^KO mice. Scale bar, 25 μm. C The disc tissues were immunoprecipitated with anti-RUNX3 and acetylation of RUNX3 was analyzed using an anti-acetyl-lysine. Data are represented as mean ± SD (n = 6). A p value of less than 0.05 was considered significant using an unpaired Student’s t-test. NS, not significant