Fig. 2

Characterization of the existence and subcellular distribution of circZNF609. A Schematic representation of the genomic location of circZNF609 together with its splicing pattern. B RT-PCR revealed that circZNF609 was amplified by divergent primers with cDNA, but not with genomic DNA (gDNA). GAPDH was used as a negative control. C The expression of circZNF609 and linear ZNF609 in MRC-5 was detected by qRT-PCR in the presence or absence of RNase R. D The RNA levels of circZNF609 and linear ZNF609 were analyzed by qRT-PCR in Actinomycin D-treated MRC-5 cells. E qRT-PCR analysis and FISH assay F were used to detect the expression of circZNF609 in the nuclear and cytoplasm of MRC-5. All data were expressed as the means ± SD of at least 3 independent experiments, *p < 0.05 and **p < 0.01