Fig. 1

CircPan3 is a conserved circRNA in the rodent cardiac tissue. A CircPan3 was generated from the 2nd–4th exons of the pan3 gene. Sequence analysis of PhyloP showed that circPan3 is conserved. B CircPan3 was amplified from the cDNA of rat cardiomyocytes by divergent primers, while it could not be amplified from gDNA. C Sanger-Seq validated the head-to-tail junction of the circPan3 sequence. D CircPan3 is resistant to digestion by RNase R. RNAs from rat cardiomyocytes were incubated with RNase R or buffer only (Mock). After digestion, the RNAs were purified. The levels of circPan3 and Pan3 mRNA were analyzed by qPCR. **P < 0.01 versus Mock. n = 3. E The expression of circPan3 in cardiomyocytes decreased upon treatment with ISO. Cardiomyocytes were treated with 10 μM ISO. The level of circPan3 was analyzed by qRT-PCR. **P < 0.01 versus 0 h. n = 3. F CircPan3 is downregulated in the murine cardiac tissues of cardiac hypertrophy. A mouse cardiac hypertrophy model was induced by treatment with ISO for 2 weeks. The level of circPan3 in mouse hearts was analyzed by qPCR. **P < 0.01. n = 6