Fig. 2

CircPan3 inhibits ISO-induced cardiomyocyte hypertrophy. A The expression level of circPan3 in cardiomyocytes transfected with circPan3-siRNA (si-circPan3) or its scramble control (si-NC) was analyzed by qPCR. *P < 0.01. n = 3. B–F Knockdown of circPan3 aggravates ISO-induced cardiomyocyte hypertrophy. Cardiomyocytes were transfected with si-circPan3 or si-NC. Forty-eight hours after ISO treatment, cardiomyocyte hypertrophy was assessed by B sarcomere organization (bar = 20 μm), C cell surface area measurement and level of D ANP, E BNP and F β-MHC. The expression level of ANP, BNP and β-MHC was analyzed by qPCR. *P < 0.05, **P < 0.01. n = 3. G The expression level of circPan3 in cardiomyocytes transfected with the circPan3 expression vector or empty vector was analyzed by qPCR. **P < 0.01. n = 3. H–L Forced expression of circPan3 inhibits ISO-induced cardiomyocyte hypertrophy. Cardiomyocytes were transfected with the circPan3 expression vector or empty vector. Forty-eight hours after ISO treatment, cardiomyocyte hypertrophy was assessed by H sarcomere organization (bar = 20 μm), I cell surface area measurement and level of J ANP, K BNP and L β-MHC. The expression level of ANP, BNP and β-MHC was analyzed by qPCR. *P < 0.05, **P < 0.01. n = 3