Fig. 6

CircPan3 inhibits ISO-induced cardiac hypertrophy by regulating the miR-320-3p/HSP20 axis. A–D CircPan3 inhibits cardiomyocyte hypertrophy by regulating miR-320-3p. Cardiomyocytes were co-transfected with the circPan3 expression vector and miR-320-3p mimic (or control mimics). Cells were treated with 10 μM ISO for 48 h. Cardiomyocyte hypertrophy was assessed by A cell surface area measurement and level of B ANP, C BNP and D β-MHC. The expression level of ANP, BNP and β-MHC was analyzed by qPCR. *P < 0.05, **P < 0.01. n = 3. E CircPan3 regulates the expression of HSP20. Cardiomyocytes were transfected with the circPan3 expression vector or empty vector and then treated with 10 μM ISO for 24 h. The expression of HSP20 was analyzed by immunoblotting. F Knockdown of HSP20 counteracts the effect of circPan3 in cardiomyocyte hypertrophy. Cardiomyocytes were co-transfected with the circPan3 expression vector and HSP20-siRNA (or its scramble control) and then exposed to 10 μM ISO for 48 h. Cardiomyocyte hypertrophy was assessed by cell surface area measurement. *P < 0.05, **P < 0.01. n = 3