Table 1 The sequences of primers used in this study
From: Circular RNA-circPan3 attenuates cardiac hypertrophy via miR-320-3p/HSP20 axis
Assay | Primer name/target | Species | Sequence (5ʹ–3ʹ) |
|---|---|---|---|
Divergent PCR | CircPan3-forward | Rat | TAGAAACAAAGTATCCCTTGATGCA |
Divergent PCR | CircPan3-reverse | Rat | TTAAAGCACCTCCATCCATTCC |
Convergent PCR | CircPan3-forward | Rat | CGGAATGGATGGAGGTGCTT |
Convergent PCR | CircPan3-reverse | Rat | ATCAAGGGATACTTTGTTTCTACCG |
qPCR | CircPan3-forward | Rat | TAAATGACAGTGCCAAGCCATAC |
qPCR | CircPan3-reverse | Rat | GCATCAAGGGATACTTTGTTTCTAC |
qPCR | Pan3-forward | Rat | TTCAGCACCAGCTTCATTGGAGTGA |
qPCR | Pan3-reverse | Rat | CCTGTGTATGGCTTGGCACTGTCA |
qPCR | METTL3-forward | Rat | GGAAGCACGCTGCCTCAGATGTT |
qPCR | METTL3-reverse | Rat | GGACTGTTCCTTGGCTGTTGTGGTA |
qPCR | METTL14-forward | Rat | TCTTCGGGAGGGACAGCACTATCAG |
qPCR | METTL14-reverse | Rat | ACGGTCAGACTTGGATTTGGGAGGA |
qPCR | FTO-forward | Rat | GGCTGTGGAAGAAGATGGAGAGTGT |
qPCR | FTO-reverse | Rat | GCTGTGCTGGTAGAGTTCGGACAA |
qPCR | WTAP-forward | Rat | CAGTACCAGCAGCAGCAGTCTCAAG |
qPCR | WTAP-reverse | Rat | CCTCCCTGTGAAATCCAGACCCAGA |
qPCR | ALKBH5-forward | Rat | ACGCAGTGACTACGAGGAGCATCA |
qPCR | ALKBH5-reverse | Rat | TGGAGCACTCATCCTGGCTGAAGA |
qPCR | GAPDH-forward | Rat | GCCCATCACCATCTTCCAGGAG |
qPCR | GAPDH-reverse | Rat | GAAGGGGCGGAGATGATGAC |
qPCR | MiR-320-3p-forward | Rat | ACACTCCAGCTGGGAAAAGCTGGGTTGAGA |
qPCR | MiR-320-3p-reverse | Rat | TGGTGTCGTGGAGTCG |
qPCR | U6-forward | Rat | CTCGCTTCGGCAGCACA |
qPCR | U6-reverse | Rat | AACGCTTCACGAATTTGCGT |
qPCR | ANP-forward | Rat | AGGAGAAGATGCCGGTAGAAGA |
qPCR | ANP-reverse | Rat | CCGAGAGCACCTCCATCTCT |
qPCR | BNP-forward | Rat | TGCTCCTGCTTTTCCTTAATCTG |
qPCR | BNP-reverse | Rat | TTGAGAGCTGTCTCTGAGCCATT |
qPCR | β-MHC- forward | Rat | AGCCTCCAGAGTTTGCTGAA |
qPCR | β-MHC- reverse | Rat | TTGATGAGGCTGGGGGTTCTG |
qPCR | CircPan3-forward | Mouse | GATCTTCTGACTTCATCTGCTTCATC |
qPCR | CircPan3-reverse | Mouse | TACTTTGTTTCTACAGGGCTTCCAA |
qPCR | GAPDH- forward | Mouse | CAGTGGCAAAGTGGAGATTGTTG |
qPCR | GAPDH- reverse | Mouse | TCGCTCCTGGAAGATGGTGAT |
qPCR | MiR-320-3p-forward | Mouse | CCAAAAGCTGGGTTGAGAGG |
qPCR | MiR-320-3p-reverse | Mouse | CCAGTGCAGGGTCCGAGGT |
qPCR | U6-forward | Mouse | CGCTTCGGCAGCACATATACTA |
qPCR | U6-reverse | Mouse | GGAACGCTTCACGAATTTGC |
qPCR | ANP-forward | Mouse | AGGCCATATTGGAGCAAATCC |
qPCR | ANP-reverse | Mouse | GCTTCCTCAGTCTGCTCACTCA |
qPCR | BNP-forward | Mouse | CTGTCCCAGATGATTCTGTTTCTG |
qPCR | BNP-reverse | Mouse | GGCCATTTCCTCCGACTTTT |
qPCR | β-MHC- forward | Mouse | GGAGGCTCTGATCTCTCAGCTAA |
qPCR | β-MHC- reverse | Mouse | GTTCCCTCAGCAGGTCACAATC |
RT | MiR-320-3p-RT | Rat | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTCGCCCTC |
RT | MiR-320-3p-RT | Mouse | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCGCCC |