Fig. 3

Detection of MYCT1 and RUNX1 mRNA, DNA and protein in lymphoma patients and the control group. A MYCT1 and RUNX1 mRNA expression in lymphoma patients with bone marrow infiltration and normal bone marrow control group. B MYCT1 and RUNX1 mRNA expression in paraffin-embedded tissues of the lymphoma and reactive lymphadenitis control group. C Representative MYCT1 FISH test results. The left figure shows the FISH results with a normal copy number of MYCT1; the figure on the right shows the FISH results of MYCT1 copy number deletion. The red signal represents the chromosome 6q25 region of the MYCT1 gene, and the green signal represents the chromosome 6p11.1-q11.11 region of the CEP6 gene. D Representative RUNX1 FISH test results. The left figure shows the FISH results with a normal RUNX1 copy number; the right figure shows the FISH results of RUNX1 copy number amplification. The red signal represents the chromosome 8q21 region, where the ETO gene is located, and the green signal represents the chromosome 21q22 region, where the RUNX1 gene is located. E MYCT1 and RUNX1 protein expression in paraffin-embedded tissues of the lymphoma and reactive lymphadenitis control group (IHC × 200) detected by immunohistochemical DAB staining. The data represent the results of three independent repeated experiments. *, * * and * * * represent p < 0.05, p < 0.01 and p < 0.001, respectively