Fig. 5

The regulatory mechanism of RUNX1 by MYCT1. A Effect of MYCT1 on the expression level of RUNX1 in the DLBCL cell lines DB and SU-DHL4. The left figure shows the effect of MYCT1 overexpression on the mRNA expression level of RUNX1 in DB and SU-DHL4 cells; the right figure shows the effect of MYCT1 overexpression on the protein expression level of RUNX1 in DB and SU-DHL4 cells. B Interaction diagram of MYCT1 and MAX predicted by STRING bioinformatics software. C Test results of binding ability between MYCT1 and MAX protein in DLBCL and HEK293T cells. The upper figure shows the application of anti-MAX antibody for immunoprecipitation. The lower figure shows the application of anti-MYCT1 antibody for immunoprecipitation. D The subcellular location of MYCT1 and MAX protein in HEK293T cells by immunofluorescence staining. E Detection results of MAX knockdown efficiency in DLBCL (mRNA level). F Detection results of MAX knockdown efficiency in DLBCL (protein level). G Effect of MAX knockdown on RUNX1 expression in DLBCL. H Schematic diagram of potential MAX binding sites in the RUNX1 promoter region. I ChIP detect results showed MYCT1 inhibits the binding of MAX to the RUNX1 promoter region. J Schematic diagram of the luciferase truncated vector containing MAX binding sites in the RUNX1 promoter region and the effect of MAX knockdown on the transcriptional activity of the RUNX1 promoter region in DLBCL cells. Data represent the results of three independent repeated experiments. *, * * and * * * represent p < 0.05, p < 0.01, and p < 0.001, respectively; NS represents no statistical significance