Fig. 2

Small RNA sequencing. A Heatmap of the correlation coefficient of the carotid artery samples. Blue represents the samples with a high correlation coefficient, and white represents the samples with no similarity. B Primary component analysis. The location of the colored dots shows the main character of the sample. The spatial distance represents the similarity of the samples. C The hierarchical clustering heatmap for tsRNAs. The color in the panel represents the relative expression level. Blue represents an expression level below the mean and red represents an expression level above the mean. D The scatter plot between the sham group and the balloon injury group for tsRNAs. Red dots (upregulated) and green dots (downregulated) indicate ≥ 1.5 fold change between the two groups. Gray dots indicate non-differentially expressed genes. E The volcano plot of tsRNAs. Red (up-regulated) and green (down-regulated) dots indicate statistically significant differentially expressed tRFs & tiRNAs with fold change ≥ 1.5 and P-value ≤ 0.05. F Venn diagram showing the number of tsRNAs expressed in the sham group and balloon injury group. G The subtypes of tsRNAs. The X-axis represents the origin of tRNAs and the Y-axis shows the number of tsRNAs. n = 3. Sh sham, BI balloon injury