Fig. 1

Overview of transcriptome and chromatin accessibility profile in different myofibers. A, B ATPase staining (A) and IHC analysis (B) of PEM and SOL in 7-week-old Xinghua chicken. C–F Heatmaps (C and E) and volcano plots (D and F) of differentially expressed mRNAs (C, D) and lncRNAs (E, F). G Pie charts showing the distribution of ATAC-seq peaks across the genome. The different colors represent different genomic regions. H Accumulated barplot showing the feature distribution of ATAC-seq peaks around the TSS. I Volcano plots of differentially expressed ATAC-seq peaks. J Gene view of coverage of lncRNA and chromatin-accessibility footprint reads on selected genes. Gene structure is diagrammed at the bottom. K Violin plot showing the changes in ATAC-seq intensity around the TSS of open chromatin-associated lncRNAs and randomly selected nondifferentially expressed lncRNAs. Results are shown as mean ± SEM. In panel K, the statistical significance of differences between means was assessed using an independent sample t-test. (**P < 0.01)